What is the primary action that occurs during Anaphase of mitosis?

During Anaphase of mitosis, the primary action is the separation and movement of sister chromatids toward opposite poles of the cell. This occurs after the chromosomes are aligned at the metaphase plate during metaphase. Specific spindle fibers, known as kinetochore microtubules, attach to the centromeres of the chromatids and, upon receiving a signal, begin to contract and pull the chromatids apart. This ensures that each new daughter cell will receive an identical set of chromosomes after the cell divides. The successful segregation of chromosomes is crucial for maintaining genetic stability and ensuring that each daughter cell has the correct number of chromosomes.